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1.1 Background to Study
Natural products, such as plants extract, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug discoveries because of the unmatched availability of chemical diversity. According to the World Health Organization (WHO), more than 80% of the world's population relies on traditional medicine for their primary healthcare needs. The use of herbal medicines in Asia represents a long history of human interactions with the environment. Plants used for traditional medicine contain a wide range of substances that can be used to treat chronic as well as infectious diseases. Due to the development of adverse effects and microbial resistance to the chemically synthesized drugs, men turned to ethnopharmacognosy. They found literally thousands of phytochemicals from plants as safe and broadly effective alternatives with less adverse effect. Many beneficial biological activity such as anticancer, antimicrobial, antioxidant, antidiarrheal, analgesic and wound healing activity were reported. In many cases the people claim the good benefit of certain natural or herbal products. However, clinical trials are necessary to demonstrate the effectiveness of a bioactive compound to verify this traditional claim. Clinical trials directed towards understanding the pharmacokinetics, bioavailability, efficacy, safety and drug interactions of newly developed bioactive compounds and their formulations (extracts) require a careful evaluation. Clinical trials are carefully planned to safeguard the health of the participants as well as answer specific research questions by evaluating for both immediate and long-term side effects and their outcomes are measured before the drug is widely applied to patients. According to the World Health Organization (WHO), nearly 20,000 medicinal plants exist in
91 countries including 12 mega biodiversity countries. The premier steps to utilize the biologically active compound from plant resources are extraction, pharmacological screening, isolation and characterization of bioactive compound, toxicological evaluation and clinical evaluation. 
Musa paradisiaca is a rich source of calcium, phosphorus, iron and some other trace elements
B. PHARMACOLOGICALLY ACTIVE COMPONENTS (UNRIPE)
The known pharmacologically active components of plantain, and the unripe type specifically can be classified into;
(i) Phenolic amines
Simmonds, (1968), reported the presence of phenolic substances in plantain. The most abundant was 3, 4 – dihydroxyethylamine (dopamine). Through dopamine is abundant in the skin of plantain, the level is insignificant in the pulp (Anderson et al, 1978). Other substances were later discovered, of which 5-Ht is the most abundant. Others include noradrenaline and tyramine.
Not much have been reported about changes in the levels these pharmacologically active substances at ripening, but 5 – HT seems fairly constant in the pulp of the fruit and increases in the skin when ripe (Simmonds, 1966).
Bucley (1966), gave the biosynthetic pathway for phenolic amine synthesis in plantain thus
BIOSYNTHETIC PATHWAY FOR PHENOLIC AMINE SYNTH ESIS IN PLANTAIN.
The pathway starts from tyrosine, which is converted to tyramine, then to dopamine, and finally to noradrenaline in plantain pulp or skin.
5-Hydroxytryptamine (5-HT) is the most abundant pharmacologically active substance present in the plantain pulp. It inhibits gastric acid secretion and stimulates smooth muscles of the intestine (Simmonds, 1966; smith et al; 1960). Simmonds, 1966, reported the usefulness of this in combating coeliac disease.
The synthetic pathway for 5-HT has not been fully elucidated, however, it is presumed to be similar to the pathway in many other animals. Tryptophan is the precursor, and it is excreted as 5-hydroxy-indoleacetic acid in mammals, after eating plantain (Simmonds, 1966).
Tannin occurs in unripe plantain (Goldstein, 1963).
Tannin is responsible for the astringent property of plantain. Astringency has been defined as the formation of tannin-protein complex in the mouth and intestine. Astringent activity leads to various buccal, gastric and intestinal secretion arrest by tannin acid-containing vegetable products (Butherworths, 1978).
In unripe plantain the bulk of tanniins is Leucoanthocyanidin which is present as a monomeric falvo – 3, 4- diol or as oligomers and is believed to condense to inactive high tannin polymers at ripening. Polymerization leads to decreased solubility of high molecule tannins in methandic solvents (Goldstein et al; 1963).
Probable site of polymerization to give an inactive condensed tannin as seen in ripe plantain (Simmonds, 1966).
Othe4r phenolic substances present in banana include histidine, Leucodelphinidin, Eugenol, elemicin
Baggosan (1932), reported the presence of phytin in plantain. Phytin is a calcium-magnesium salt of phytic acid has been shown to interfere with the absorption of calcium either by precipitation or by converting it to a form which is not readily absorbed from the intestine. (Anon, 1945). Inositol is a basic sugar alcohol called myoinositol and is present in the form of phosphatidly inositol (Phytic acid) in the extracellular compartment of higher plant tissues (Lehninger, 19677).
Phytic acid has been shown to produce a negative calcium balance by binding as non-ionizable complex thereby reducing absorption considerably (Anon, 1945).
1.4 ECONOMIC/MEDICINAL USES OF Musa paradisiaca
Musa paradisiaca have been associated with antispasmodic action and implicated in antidarrhoeal management. There are other myriad of pharmacological actions associated with plantain, hence its use in diverse conditions, in addition to being a source of food for man.
The medicinal uses of Musa paradisiaca. Vary from locality to locality.
The tradition applications and formulations have been severely criticised for lack of: standardization of its dosage regimen and specification of quantities related to the age or body weight. In spite of these setbacks, it has continued not only to provide modern medicine with a numbers of compounds, either useful in themselves or capable of improvement by chemical modification, but also has continued to meet the health care needs of the people, especially, in the developing countries.
Musa paradisiaca also makes a delicious meal. It is consumed in large quantity in the coastal regions of West Africa (William et al; 1980). Generally, the demand for plantain in Nigeria is relative low. However, it is one of the commonest food in the Niger Delta region (Oyenuga, 1968).
The pulp has high caborific value due to the high carbohydrate content. The pulp contains mainly simple sugars-fructose and glucose (Jay; 1970; Coursey; 1967).
The fruit is prepared by roasting; boiling; frying in oils; mashed into paste as fufu; or smoked for storage till when needed for use. The plantain skin when dried under the sun is a good materials to provide ash, rich in potash for soap making (Dalziel; 1936). The use of plantain for beer brewing has also recently been reported in some parts of Tanzania and Uganda.
The sap of plantain produces indelible stain in fabrics and can be stalk of the plant is pounded and smeared on the floor of native mud houses. Plantain also provides a good fibre for making ropes and brushes, from the peduncles.
1.5 RELEVANT PHYSIOLOGICAL & PHARMACOLOGICAL PROCESSES AND CONCEPTS.
The ileum like other parts of the small intestine consists of four histological layers. These are from outside, the serosa layer, the muscular layer, the submucosa, and the mucosa layer.
The serosa layer is a continuation of the mesentery and is tightly bound to the underlying muscle layer. The muscular layer consists of outer longitudinal layer and an inner circular layer. The tow muscular layers are separated at some points by the dysenteric nerve plexus. Stimulation of this plexus causes contraction of the muscular layers producing peristaltic wave along the entire gastrointestinal tract. The subnucosa consists of areolar tissue with blood and lymph vessels and the meissner’s nerve plexus. The mucosa consists of finger-like folds or villi which are modified for digestion and absorption of food substances.
COURTESY, VAN DAN BROUCHE & LEMLI, 1980)
The smooth muscle layer of ileum is innervated by post-ganglionic fibre located in the plexus which is derived from both the sympathetic and parasympathetic divisions of the post-ganglionic parasympathetic fibres with the liberation of cholinergic nero transmitter acetylcholine leads to increase in the motility and tone of the gut while stimulation of the sympathetic fibres causes moradrenaline release which causes a decrease in the tone and motility of the ileum.
An indept review of the gastro-intestinal physiological provides an insight into the general principles of intestinal motility which will help in laying a good foundation for a better understanding of interstinal motility both vivo and invitro. It will also help to appreciate the effects of drugs on the gastro-intestinal tract. Hence, sometime will be devoted to this topic.
The smooth muscle contains both actin and Myosin, the contractile proteins. Smooth muscle also contains tropomyosin but is is doubtful whether troponin or a tropomyosin-like substance exists in smooth muscle (Guyton, 1981). On stimulation of the muscle, there is an interaction between actin and Myosin, similar to that found in skeletal muscle. Furthermore, the contractile process is activated by calcium ions, and adenosine triphosphate (ATP) is reduced to adenosine diphosphate (ATP) to provide energy for the contrition.
The actual contractile process in skeletal and smooth muscles is activated by calcium ions. However, the source of calcium ions differ in smooth muscle bacuse the sarcoplasmic reticulum of skeletal muscle which is extensive and is the source of very nearly 100% of the contraction- inducing calcium ions. Due to the poor development of darcoplasmic reticulum in smooth muscle, most of the calcium ions used in contraction are obtained from extracellular fluid at the time of depolarization (Guyton, 1981). The lack of extracellular calcium or the intracellular blockade of calcium due to one factor or the other in smooth muscle results in muscle relaxation. Lack of calcium ion leaves the actin-nyosin fibres, cannot contractive state. The smooth muscle therefore, cannot contract (Hurwitz et al; 1972).
The gastro-intestinal tract is innervated by intrinsic and extrinsic nerves. The intrinsic innervation is the mycenteric and meissners plexuses. The extrinsic is the parasympathetic and sympathetic nervous supplies. The parasympathetic and sympathetic nervous supplies. The para-sympathetic nerves are stimulatory while the sympathetic nerves are inhibitory to the gastro-intestinal smooth muscles and
The autonomic control of gastro-intestinal functions is not only exerted through adrenergic or cholinergic nerve fibres but also through inhibitory purinergic nerves with unknown postoganglionic neuro transmitters (Martinson, 1960). From the above discussion, it is possible that motility of the ileum can be modified by stimulating either the parasympathetic or sympathetic systems.
SPASMOGENS AND SPASMOLYTICS
Spasmogens are substances that can increase the contractility of smooth muscles. They are agonists. The spasmogens can act either at the receptor sites on the muscle membrane or on the nervous supply. Specialized and different receptor sites on the cell surface appear to be involved in response to each mediator. The selectivity of each of these types or receptors for certain types of chemical structures and the competitive kinetics between the structurally related substances which are often found, suggest that the ability to interact with each of receptor site is determined by its chemical structure (Daniel, 1964), fig 1.8 summarizes the sites of some drug effects in the guinea-pig ileum.
This discussion is useful in that by relating the different spasmogens with different action mechanisms, information on the pharmacological mechanism of action plantain extract can be revealed, especially when the effects of the extracts are related to the effects of the spasmogens.
Acetylocholine the acts directly on the smooth muscle by activating the cholinergic receptors. This activation is linked to CGMP. The acetylcholine-receptor interaction has been associated with increases release and depressed uptake of 42k+ in the muscle cells. (A.K Banergic and J.J Lewis, 1964). There is also evidence for a slight indirect action of acetylcholine, perhaps, through stimulation of ganglion cells (Feldbergy, 1951).
Histamine acts directly on the smooth muscle cells of the guinea-pig ileum (Kosterlitz & Robinson, 1958). Banerjee and J.J Lewis (1964), also reported increase in 42k+ release and depressed influx, associated with action of histamine. In the guinea-pig ileum (Gaddum & Pi carelli, 1957): the M receptors which are blocked by morphine and the D receptors which are blocked by phenoxybenzamine. The M receptors are generally assumed to be in nervous tissue and this is assumed to polay the major role in guinea-pig ileum contractility, while on the other hand, D receptors are found on the smooth muscles. 5 – HT also increase release and depresses uptake of 42k+ in smooth muscles. It has been suggested that 5-HT and 1, 1-dimethylphenyl-piperazinim activate different nerve-pathways; the acetylcholine released by 5-HT being hydrolysed mostly by acetylkcholinesterase whereas that released by dimethyphenyliperazinium, and also exogenous acetycholine is hydrolysed by a mixture of both butyryl and acetylcholinesterases (E.S. Johnson, 1964).
Nicotine; 1, 1-dimethylphenyl piperazinium, tetramethylammonium are all indirect spasmogens (Day and Vane, 1964). Other agents which are known to cause contraction of the gastro-intestinal tract include Ca2+, Ba2+ and Kcl.
Atropine and related compounds, hyoscine and homatropine have been recommended and employed in a wide variety of conditions known or supposed to involve increased tone ‘’spasticity’’ or motility of the gastro-intestinal tract. Their antagonism of the muscarinic actions of acetylcholine is the basis of most, but not all, of their pharmacological actions. The ant muscarinic effect is associated with depressed release of 42k+ due to acetycholine (Banerjee & Lewis, 1964).
Other spasmolytic agents do so through interference with the role of calcium in excitation contraction coupling, like papaverine.
Inhibitors of oxidative phosphorylation also produce spasmolytic action, like papaverine (Ferrari, 1963).
The phosphodiesterase inhibitors like xanthines also make another group of spasmolytic agents.
1.6 DIARRHOEA AND DIARHOCAL THERAPEUTIC STRATEGIES
One of the most important clinical uses of antispasmodic agents is in the treatment of diarrhoea. Therefore, this introduction might not be complete if a brief discussion on diarrhoea is not made.
Diarrhoea is an increased loss of water and electrolytes, and is characterized by a stool weight greater than 200g per day. Diarrhoea may occur alone or in associated with increased fecal loss of other nutrients such as fat (Steatorrhea) or nitrogen (azo torrhea).
The increased fecal loss occur when the absorptive load exceeds the absorptive capacity. This can be caused by;
(1) Increased absorptive load: This occurs in
(a) High intake of food, like in excessive amount of tubne feeding. The high quantity of food may then start playing a kind of osmotic cathartic role
(b) Decreased absorption: This is caused by intramural digestive disturbances, involving the mucosa or enzymes.
In the light of the above discussion, different approaches have been adopted for the treatment of diarrhoea. It is worthy of note that drugs prescribed for diarrhoea are determined on the cause or causation. Diarrhoea, therefore, may result from the following:
(a) Eating of contaminated or partially decomposed food.
(b) Bacterial or protozool infection.
(c) Due to nervous disorder in GIT.
(d) Due to inflammation of the intestine or. Adjacent viscera.
Diarrhoea may at times produce very serious consequences, for example, dehydration; exhaustion; loss of electrolytes, vitamins and food materials, hence need arises for medical intervention by the following means.
1. Demulcnets and protective e.g salts of bismuth.
2. Adsorbents e.g… Activated charcoal, chalk mixtures.
3. Astringents e.g. Tannin.
4. Antiseptics e.g. Antibiotics.
5. Antispasmodics eg. Atropine, opiates. The antispasmodics are contra-indicated in diarrhoea caused by infections, such as bacillary dysentery, where by diarrhoea is an adaptive mechanism to evaluate the irritated intestine of the infected contents.
6. Oral rehydration solution of water, salt and sugar.
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