Get the complete project »
- The Complete Research Material is averagely 112 pages long and it is in Ms Word Format, it has 1-5 Chapters.
- Major Attributes are Abstract, All Chapters, Figures, Appendix, References.
- Study Level: BTech, BSc, BEng, BA, HND, ND or NCE.
- Full Access Fee: ₦7,000
Multi-drug resistant tuberculosis (MDR-TB) remains a major challenge for the control of
tuberculosis worldwide. Susceptibility testing of Mycobacterium tuberculosis isolates to first line drugs is therefore necessary to ascertain the diagnosis of MDR-TB. A total of 437 re-treatment patients’ samples were screened for Acid Fast Bacilli (AFB), 72 were smear positive giving a prevalence of 16.47%. Out of 72 smears positive, 62 were culture positive, 6 were culture negative and 4 were contaminant using Lowenstein Jensen medium. Out of 62 cultures positive, 57 were found to be Mycobacterium tuberculosis complex (MTBC) using immunochromatographic test while 5 were negative which were considered to be NTM (Non-Tuberculosis Mycobacteria). In this study the susceptibility of 57 MTBC isolates to isoniazid (INH), rifampicin (RIF), streptomycin (STR) and Ethambutol (EMB) was determined by Lowenstein Jensen proportion method (LJPM) and Nitrate Reductase Assay (NRA). LJ PM detected 41(71.93%) MDR-TB while NRA detected 44(77.19%) MDR-TB isolates. The occurrence of poly resistance to anti-TB drugs using NRA and LJ PM was 12% and 14% while pan susceptible were of 11% and 14% by LJ PM and NRA respectively. The sensitivities and specificities of NRA compared to those of LJPM were observed to be 98% and 98%, 64% and 68%, 89% and 92%, 80% and 77% for RIF, INH, STR, and EMB respectively. Positive predictive values were 91%, 93%, 87% and 83% for RIF, INH, STR and EMB respectively. Negative values were 80%, 92%, 67% and 90% for RIF, INH, STR and EMB respectively. Good agreement was found in all the tests with κ values of 0.63, 0.61, 0.59, and 0.61for RIF, INH, STR, and EMB respectively. The HIV/TB co-infections and HIV/MDR-TB co-infections were reported to be 9.7% and 7% respectively. Highest MDR-TB among age groups of 21-30 and 31-40 years were detected by NRA and LJPM and higher MDR-TB and TB were observed in male. The NRA has the potential to be a useful tool for rapid detection of MDR-TB in resource-limited settings because of its higher sensitivity and specificity.
1.1 Background of the Study
Tuberculosis (TB) continues to cause more deaths worldwide than any other infectious disease.
The World Health Organization (WHO) estimated that in 2011, there were 8.7 million cases of
TB, with nearly 1 million deaths among HIV-negative cases and 0.43 million deaths associated
with HIV infection (WHO, 2012). More than 99% of deaths and 95% of new TB cases occur in
middle- and low-income countries (Palomino, 2012). In the 2012 Global Tuberculosis Report
estimates, expressed in rates per 100,000 populations, were 161 (25-420) for prevalence and 108
(50-186) for incidence. Case detection of all forms stood at 51% (29%-110%). The mortality rate
for all forms of TB remains 27 (7-60) per 100,000 populations (46,000 deaths per year) (WHO,
2012). The majority of TB cases worldwide were in the South-East Asia (29%), African (27%)
and Western Pacific (19%) regions. India and China alone accounted for 26% and 12% of total
You either get what you want or your money back. T&C Apply
You can find more project topics easily, just search
SIMILAR MICROBIOLOGY FINAL YEAR PROJECT RESEARCH TOPICS
1. PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY OF GRAM NEGATIVE BACTERIA IN THE URINE OF CARITAS UNIVERSITY STUDENTS» CHAPTER ONE INTRODUCTION Gram negative bacteria are bacteria that do not retain their crystal violet dye in the gram staining protocol. They are diffe...Continue Reading »
2. DETOXIFICATION AND ANTI-NUTRIENT REDUCTION OF JATROPHA CURCAS SEED CAKE BY FERMENTATION USING BACILLUS SPECIES» ABSTRACT Jatropha curcas seed cake is a by product generated from the oil extraction of J. curcas seed a biodiesel producing plant‘s seed. Altho...Continue Reading »
» ...Continue Reading »
» TABLE OF CONTENTS Pages Title Page i Certification ii Dedication iii Acknowledgements iv Table of Contents v List of Tables x List of Figures xi Abstr...Continue Reading »
» CHAPTER ONE 1.0 INTRODUCTION Ugba also called ukpaka is a popular food delicacy in Nigeria especially among Ibo ethnic group. It is rich in protein an...Continue Reading »
» TABLE OF CONTENTSCHAPTER ONE1.0 Introduction 1.1 Statement of the problem 1.2 Aims/Objective of the Study 1.3 Limitation of the Study 1.4 Significance...Continue Reading »
» CHAPTER ONE INTRODUCTION Background to the Study Eggplant (Solanum melongena L.; Division Anthophyta; Class Dicotyledoneae; Order Solanales) is an agr...Continue Reading »
8. PHYSIOCHEMICAL AND SENSORY EVALUATION OF COOKIES PRODUCED FROM BLENDS OF MALTED SORGHUM, SPROUTED BLACK BEANS AND COOKED COCOYAM FLOUR, PROXIMATE ANAL...» CHAPTER ONE 1.1 BACKGROUND OF STUDY Over the years the production of cookies of various types has improved with the advancement of new technology in m...Continue Reading »
9. COMPARATIVE STUDY OF ANTIBACTERIAL ACTIVITY OF TWO SELECTED MEDICATED SOAP AND ONE LOCAL BLACK SOAP ON STAPHYLOCOCCUS AUREUS FROM WOUND INFECTION» ABSTRACTTwo medicated soaps and one local soap were investigated for their antibacterial activities against Staphylococcus aureus. Identification of t...Continue Reading »
10. THE IDENTIFICATION OF PREVALENCE MICROBES ASSOCIATED WITH FROZEN FISH MARKETED IN OWERRI METROPOLIS» ABSTRACT Aquaculture products can harbour pathogenic bacteria, which are of the natural micro flora of the environment. An in vitro assay was carried ...Continue Reading »