Get the complete project »
- The Complete Research Material is averagely 61 pages long and it is in Ms Word Format, it has 1-5 Chapters.
- Major Attributes are Abstract, All Chapters, Figures, Appendix, References.
- Study Level: BTech, BSc, BEng, BA, HND, ND or NCE.
- Full Access Fee: ₦4,000
Ugba also called ukpaka is a popular food delicacy in Nigeria especially among Ibo ethnic group. It is rich in protein and is obtained by a solid state fermentation of the seed of African oil bean tree (Pentaclethra macrophylla Benth). It is essential food item from various traditional ceremonies where it is mixed with slices of boiled stock fish (ugba and okpoloko). The natural fermentation of the seed which at present is still done at the house-hold level, renders the production nutritious, palatable and non-toxic (Enujiugha, 2002).
Its production, like many African fermented foods depends, entirely on mixed fermentation by microorganism from diverse source.
Some of these seeds have been exploited as soup bases such as Gbegiri from Vigna unguicuata (Akanbi, 1992). Others are fermented and used as condiments and seasonings such as okpei from Prosopis africana (Achi, 1992) and ogiri from Ricinus cummunis (Odunka, 1989). Another of such seeds is the African oil bean seed (Pentaclethra macrophylla Benth), a highly nutritious leguminous crop seed abundant in the rain forest areas of west and central Africa.
Pentaclethra macrophylla Benth is a large woody plant abundant in the rain forest areas of west and central Africa. It’s origin in Nigeria is believed to be around 1937 (Ladipo, 1984); where it is found in the South Nigeria, (Mbajunwa et al, 1998).
“Ugba” Pentaclethra macrophylla Benth belongs to the Family Leguminosae and sub-family microsoideae (Keay, 1989 and NFTA, 1995).
Ugba seeds are irregular and oval, they are flat, black and hard pods. It is composed of oil, protein and small amounts of carbohydrate (Obeta, 1982).
Production of Ugba is still on age old traditional family in the rural area. The fermentation depends on random inoculation of boiled slices of the oil bean seed by microorganism within he immediate environment. Earlier report attribute the sources of the micro-organism to the leaves used in packaging, human handling, container and utensils used in processing (Obeta, 1993, Odunfa & Oyeyiola, 1985).
A number of researches have been carried out to know what causes this fermentation, and was attributed to micro-organisms that are probably introduced through the air, water, and banana leaves. Organisms such as Micrococus roseus, Micrococcus luteus, Leuconostoc mesenteroides, Staphylococcus aureus, Staphylococcus epidermidis, Bacillus substilis, Bacillus megaterium and Bacillus circclans, E-Coli
Fermented African oil bean seed (ugba), has a high rate of susceptibility to microbial spoilage and therefore has a very short self life of 1-2 weeks (Enujiugha & Olajundoye, 2001).
Manifestation of food spoilage are many and vary typically resulting in an off smell, colour, taste and texture.
Reports by Mbajunwa (1998) Obeta, (1983) and Nwagu et al. (2010) indicates that micrococcus species do not play an active role during microbial fermentation of Ugba.
However, further work on spoilage association of ugba by Nwagu et al (2010) showed that the population of Micrococcus sp increased with increase in keeping time of ugba. This indicates the ability of micrococcus to thrive in the alkalophilic environment while constituting as a spoilage organism of ugba. This may be attributed to the ability of microorganism to produce lipase, lipase, or protease able to utilize protein, carbohydrate or lipid content of ugba as source of nutrition (Njoku et al., 1990).
Lipase are defined as glycerol ester hydrolases (EC18.104.22.168) hydrolyzing tri-di and mono-glycerides present at oil-water interface (Saxena et al, 1999). Some lipases are also able to catalyze esterification, trans-esterification and enantioselective hydrolysis reaction (Nine et al, 2001, Shintre et al, 2002). The interest in microbial lipase production has increased in the last decades, because of its large potential in a wide range of industrial applications and additives in food processing (flavour modification).
1.1 Aims and Objectives
This work is aimed at the following;
a. isolating micrococcus from fermented ugba
b. producing lipase from the micrococcus from ugba
You either get what you want or your money back. T&C Apply
Share a Comment
You can find more project topics easily, just search
SIMILAR MICROBIOLOGY FINAL YEAR PROJECT RESEARCH TOPICS
» CHAPTER ONE1.0 INTRODUCTIONNewcastle disease (ND) is one of the most important avian viral diseases because of its high economic impact on the poultry...Continue Reading »
2. ANTIMICROBIAL ACTIVITY OF VINEGAR, HONEY, BLACK PEPPER, GINGER AND MUSHROOM EXTRACT ON WOUND ISOLATE» Abstract Suspensions of vinegar, honey, black pepper and ginger were examined for antibacterial activity against Salmonella sp., Pseudomonas sp. and E...Continue Reading »
» CHAPTER ONE 1.0 INTRODUCTION Ugba also called ukpaka is a popular food delicacy in Nigeria especially among Ibo ethnic group. It is rich in protein an...Continue Reading »
» Abstract This study was carried out on the microbiological analysis of dry cassava peel used in the preparation of pigs feed. Since the nutritional co...Continue Reading »
5. ASSESSMENT OF EXTRACELLULAR BETA-GALACTOSIDASE PRODUCTION POTENTIAL OF FUNGI ISOLATED FROM DAIRY EFFLUENT» ABSTRACT Dairy wastewater is increasingly becoming an environmental concern. There is a widespread interest in the use of beta galactosidase (EC 3.2.1...Continue Reading »
6. EVALUATION OF THE EFFECTS OF ANTHROPOENIC ACTIVITIES AND DOMESTIC WASTE ON SURFACE WATER QUALITY (A CASE STUDY OF RIVER NIGER IN KATCHA L.G.A, NIGER)...» CHAPTER ONE 1.0 INTRODUCTION 1.1 Background of the Study Water is essential for life. Man needs water for various other purposes apart from drinking a...Continue Reading »
» CHAPTER ONEINTRODUCTIONChlamydiae are small gram negative obligate intracellular microorganisms that preferentially infect squamocolumnar epithelial c...Continue Reading »
8. ASSESSMENT OF AFLATOXIN M1 AMONG LACTATING MOTHERS AND THEIR INFANTS IN DAMATURU YOBE STATE NIGERIA» ABSTRACT Aflatoxin M1 is a biomarker of aflatoxin B1 which is detected in breast milk, a possible risk factor for infant early exposure to Aflatoxin. ...Continue Reading »
» ABSTRACT This study was aimed at investigating the microbial load and the quality of indoor air Faith Mediplex Centre, Benin City, to ascertain their ...Continue Reading »
10. A SURVEY OF THE ANTIBACTERIAL ACTIVITY OF THYMUS VULGARIS AND MYRISTICA FRAGRANS ON STREPTOCOCUSS PNEUMONIAE AND PSEUDOMONAS AERUGINOSA» ABSTRACT The aqueous and ethanolic extracts of the leaves of Thymus vulgaris (thyme) and the seeds of Myristica fragrans (nutmeg) were screened for th...Continue Reading »