- The Complete Research Material is averagely 52 pages long and it is in Ms Word Format, it has 1-5 Chapters.
- Major Attributes are Abstract, All Chapters, Figures, Appendix, References.
- Study Level: BTech, BSc, BEng, BA, HND, ND or NCE.
- Full Access Fee: ₦6,000
Get the complete project »
![Download Code Ready Instant Download Active](https://codemint.net/assets/home/instantdownloadMain.png)
ABSTRACT
This study was aimed at investigating antitrypanosomal activities and haematological profile
of crude extract and fractions of the stem bark of Acacia nilotica and Khaya senegalensis
plants against Trypanosoma brucei brucei infected Wistar rats with a view to determining the
phytochemical constituents and LD50 of Acacia nilotica and Khaya senegalensis,
antitrypanosomal activities of crude extract of Acacia nilotica and Khaya senegalensis
against Trypanosoma brucei brucei infected Wistar rats, antitrypanosomal activities of the
plant fractions administered to Trypanosoma brucei brucei infected Wistar rats and
haematological profile of Trypanosoma brucei brucei infected Wistar rats, before and after
administration of crude extract and fractions. The phytochemical constituents and toxicity of
the stem bark of both plants were determined by the standard method and the
LD50respectively. The methanolic extracts and fractions of the plants was administered to the
Wistar rats intraperitoneally daily and the parasitaemia count was determined using the rapid
matching method. PCV, WBC and differential counts were determined before and after the
administration to ascertain any significant differences. The phytochemical constituents of the
stem barks of Acacia nilotica and Khaya senegalensis crude extracts and fractions revealed
the following secondary metabolites; Alkaloids, tannins, glycosides, cardiac glycosides,
saponins, triterpene, carbohydrates and flavonoids. The LD50 for the crude extract of the stem
bark of Acacia nilotica was 707.1mg/kg body weight while the LD50 for the fractions (N-
hexane, ethyl acetate and N-butane) was 547.7 mg/kg, 387.3 mg/kg and 707.1 mg/kg body
weight respectively. The LD50 for the crude extract of the stem bark of Khaya senegalensis
was 547.7mg/kg body weight while the LD50 for the fractions (N-hexane, ethyl acetate and
N-butane) was 387.3 mg/kg, 547.7 mg/kg and 223.6 mg/kg body weight respectively. The
stem barks of Acacia nilotica and Khaya senegalensis crude extracts (100, 200, 300 and
400mg/kg body weight) and fractions (50, 100, 150 and 200mg/kg body weight) had
vi
antitrypanosomal activity. Parasites were cleared from circulation within 12 days of
treatment. Haematological indices of Acacia nilotica and Khaya senegalensis in
Trypanosoma brucei brucei infected Wistar rats showed that there was no statistical
significant change in the packed cell volume, white blood cells and differential counts before
and after treatment with all doses of the crude extracts and fractions. The findings in this
study provide very useful source for biopharmaceutical industries for the development of
antitrypanosomal agents from the stem bark of Acacia nilotica and Khaya senegalensis for
therapeutic intervention in the control of African trypanosomiasis. There is need for further
extensive work on these plants using different Trypanosoma species in the management of
African trypanosomiasis.
CHAPTER ONE
1.0 INTRODUCTION
African Trypanosomiasis (African sleeping sickness) is caused by trypanosomes which is
found in Sub-Saharan Africa and is threatening more than sixty million lives on daily
basis (Abdullahi and Emmanuel, 2012). Trypanosomes are protozoan parasites in the
family Trypanosomatidae. Most trypanosomes are transmitted by the vector, tsetse flies
(Glossinia spp) which are found only in Sub-Saharan Africa, between latitudes 14o N and
20o S (Bernard and Alain, 2012). The parasites include Trypanosoma brucei gambiense
and Trypanosoma brucei rhodesiense, (cause Human African Trypanosomiasis). Other
trypanosomes primarily affect animals include Trypanosoma congolense, Trypanosoma
vivax, Trypanosoma brucei brucei, Trypanosoma simiae and Trypanosoma godfreyi
(Bernard and Alain, 2012).
Nigeria‟s natural habitation is made up of both savannah and tropical rainforest, which
falls within the endemic area in Africa i.e. between latitude 150Nand 290 S. The diverse
flora offers a wide spectrum of unique medicinal plants. There are varieties of studies of
Nigerian plants used in the traditional management of trypanosomiasis, indicating
significant anti-trypanosomal activity (in-vitro/in-vivo); some of which the metabolites
responsible have been isolated and reported (Atawodi et al, 2003).
Trypanosoma brucei brucei are unicellular parasites transmitted by the tsetse fly. They
are the causative agent of African animal trypanosomosis (AAT), also known as Nagana.
Trypanosoma brucei brucei is the etiological agent for sleeping sickness which is one of
the most serious protozoan diseases in Africa (Antia et al., 2009; Simarro et al., 2011).
You either get what you want or your money back. T&C Apply
![](https://codemint.net/assets/public/img/100-money-back-guarantee.png)
You can find more project topics easily, just search
-
SIMILAR MICROBIOLOGY FINAL YEAR PROJECT RESEARCH TOPICS
-
1. OPTIMIZING ALPHA AMYLASE PRODUCTION FROM LOCALLY ISOLATED Aspergillus species USING SELECTED AGRO WASTES AS SUBSTRATES
» ABSTRACT α Amylase are enzymes that are used in the degradation of starch. This study focuses on optimizing the production of α Amylase us...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
-
2. EFFICACY OF ARTESUNATE IN THE TREATMENT OF URINARY SCHISTOSOMIASIS AT UMUIKWU- ANAM COMMUNITY IN ANAMBRA WEST LOCAL GOVERNMENT AREA OF ANAMBRA STATE, ...
» ABSTRACT A study on the efficacy of artesunate in the treatment of urinary schistosomiasis at Umuikwu Anam community, Anambra West Local Government ar...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
-
3. MYCOFLORA OF STORED LOCUS BEANS (Parkia biglobosa ) PURCHASED FROM USELU MARKET
» ABSTRACT Stored locust bean samples were purchased from Uselu market and analysed for their fungal load and physicochemical properties such as moistur...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
-
4. ONION IS ASSOCIATED WITH MICRO-ORGANISMS WHICH ARE CAPABLE OF CAUSING SPOILAGE.
» ABSTRACT Onion is associated with micro organisms which are capable of causing spoilage. Onions with rots were examined microscopically. A solution of...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
-
5. INVESTIGATION OF ANAEROBIC PROCESSES IN SEPTIC TANK AS A WASTEWATER TREATMENT OPTION
» CHAPTER ONE INTRODUCTION 1.1 BACKGROUND The septic tank system is the most widely used onsite treatment system for domestic wastewater. In fact, most ...Continue Reading »Item Type & Format: Project Material - Ms Word | 95 pages |
Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
-
6. DEVELOPMENT OF WASTE BASED BIOSTIMULANT FOR THE REMEDIATION OF CRUDE OIL CONTAMINATED SOIL
» ...Continue Reading »Item Type & Format: Project Material - Ms Word | 60 pages |
Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
-
7. ISOLATION AND IDENTIFICATION OF AIR MICROFLORA IN MICROBIOLOGY LABORATORY
» ABSTRACT Microflora contamination in laboratories and hospitals is becoming a serious problem worldwide, and the characterization of such contaminants...Continue Reading »Item Type & Format: Project Material - Ms Word | 62 pages |
Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
-
8. ASSESSMENT OF NEWCASTLE DISEASE VIRUS ANTIBODIES AMONG BIRDS REARED
» CHAPTER ONE1.0 INTRODUCTIONNewcastle disease (ND) is one of the most important avian viral diseases because of its high economic impact on the poultry...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
-
9. PRODUCTION AND QUALITY EVALUATION OF BANANA (MUSA SAPIENTUM) WINE
» BSTRACTJuice was extracted from banana (Musa sapientum) pulp with the addition of lemon juice and was inoculated with Baker’s yeast (Saccharomyces c...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
-
10. THE PRODUCTION AND STUDY OF SHELF LIFE OF YOGURT WITHOUT PRESERVATIVE.
» CHAPTER ONE 1.0 INTRODUCTION Yogurt is a fermented milk product that contains the characteristics of bacterial cultures lactobacillus bulgaricus and s...Continue Reading »Item Type & Format: Project Material - Ms Word | 25 pages |
Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT