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ABSTRACT
Analysis of proximate composition, mineral composition, heavy metals concentration,
microbiological counts and identification of microbial contaminants were carried out
in beef and Kilishi samples that were produced and collected from six production
spots in Kaduna state, Nigeria viz: Sabon Gari, Tudun Wada, Zaria city, Malali,
Anguwan Sarki and Kasuwa. Also, Kilishi that was prepared in the Laboratory that
served as control was analyzed. Results of proximate composition showed that Kilishi
is richer in nutrients. It showed that the profile proximate parameters in the study
samples is protein > crude fat > ash > moisture > carbohydrate > crude fibre. The
highest protein content found was in dried beef. From the study samples, Kasuwa
Kilishi had the highest protein content (64.28%), Malali Kilishi had the highest
moisture and fat contents (9.25 and 22.42% respectively), while Anguwan Sarki
Kilishi had the highest ash content (9.42%). Also, Tudun Wada Kilishi had the highest
crude fibre content (3.42%), Zaria City Kilishi had the highest carbohydrate content
(8.32%), while the Laboratory Kilishi that served as control recorded the lowest
percent moisture (7.22%) and had lower values of protein and carbohydrate. Results
of mineral composition showed that Kilishi contained the highest contents of Na, P
and K while the concentrations of Ca and Mg found were highest in dried beef.
Kasuwa Kilishi had the highest contents of Na (8281.00) and Mg (474.55) compared
to other Kilishi samples. The highest values of P (8915.00) and K (9610.00) obtained
were from Malali Kilishi, Tudun Wada Kilishi had the highest Ca content (459.55),
while the Laboratory Kilishi had the lowest mineral contents of Ca, Mg and P. The
results revealed that Kilishi contained the highest concentrations of the heavy metals.
The concentrations (mgkg-1) of Fe (142.18 - 192.80), Zn (80.09 - 135.43), Pb (1.83 -
13.75) and Ni (2.08 - 19.88) in this study exceeded their threshold effects levels
vi
(80.00, 50.00, 0.50 and 0.50 mgkg-1/day respectively). From the study samples, Co
and Mn contents (0.00 - 0.68 and 10.95 - 19.50 respectively) exceeded their threshold
effects levels (0.16 and 11.00 mgkg-1/day respectively); except in Kilishi samples
from Sabon Gari and Zaria City were Co was below detection limits. However, Cd
and Cr were below detection limits in all the samples, while Cu contents (2.84 - 7.58)
in the entire sample were below the threshold effects levels 8.0 mgkg-1/day). The
Laboratory Kilishi had Co and Mn contents that were below threshold effects levels.
Tudun Wada Kilishi had the highest Fe value, Sabon Gari Kilishi had the highest Zn
value, Zaria City Kilishi had the highest Pb value, while Sabon Gari Kilishi had the
highest Ni value. Microbiologically (cfu/g), Staphylococcus and fungi counts in all
the samples (1.56 × 105 - 3.63 × 107 and 1.01 × 104 - 2.02 × 105 respectively) were
above WFP recommended limits 106 and 102 respectively. Coliforms counts (0.00 -
9.61 × 106) were above WFP threshold limits in some of the study samples. However,
the Mesophillic aerobic counts (0.00 - 9.03 × 105) were below the threshold effects
limits of 106 with Tudun Wada Kilishi having the highest value. Microbial
contaminants of Staphylococcus aureus, Bacillus spp., Salmonella, Klebsiella,
Escherichia col
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