- The Complete Research Material is averagely 116 pages long and it is in Ms Word Format, it has 1-5 Chapters.
- Major Attributes are Abstract, All Chapters, Figures, Appendix, References.
- Study Level: MTech, MSc or PhD.
- Full Access Fee: ₦10,000
Get the complete project »
ABSTRACT
Type 2 diabetes (T2D) occurs when there is an advanced determent in insulin action (insulin resistance, IR), which proceeds toβ-cell dysfunction. This present study assessed the modulatory effects of Buchholzia coriacea (B. coriacea) seeds extract in high fructose-fed, streptozotocin-induced T2D in male Wistar rats.
Methanolic extract (MEBC), hexane fraction (HFBC), ethyl acetate fraction (EFBC) and n-butanol fraction of BC (BFBC) were prepared using 70% methanol and successive solvent partitioning method respectively. Antioxidant activities of 1-1-diphenyl 2-picryl hydrazyl (DPPH), nitric oxide radical scavenging assay (NOSA) and hydroxyl radical scavenging activities (HRSA) were assessed as well as α-amylase inhibition in vitro. High fructose (20%, p.o) (2 weeks) followed by streptozotocin (STZ) (40 mg/kg, i.p.) (FRU + STZ) (day 14) administered to achieve T2D in vivo. Control normal and diabetic untreated (FRU + STZ) rats were administered carboxymethyl cellulose (CMC) (1 ml/kg, p.o). Diabetic treated rats received BFBC (20, 200, 400 mg/kg, p.o), metformin (7.14 mg/kg, p.o) and glibenclamide (0.07 mg/kg, p.o) respectively.
BFBC had the highest percentage yield, DPPH and α-amylase inhibition activities, although, EFBC had a better inhibitory activities on HRSA and NOSA respectively. Also, untreated diabetic rats showed increase (p< 0.05 - 0.001) in blood glucose levels (BGLs), insulin (6 folds) and lipid peroxidation (LPO) levels in pancreas when compared with normal group. BFBC (20, 200, 400 mg/kg) showed decrease (p< 0.05) in BGLs in a time dependent manner in the BFBC treated animals.Similarly, BFBC produced a dose dependent decrease in serum insulin levels by 51% (20 mg/kg), 54% (200 mg/kg) and 70% (400 mg/kg) respectively.These effects were also comparable to metformin and glibenclamide. BFBC treatments elevated (p> 0.05) high density lipoprotein, but decreased (p> 0.05) triglycerides, total cholesterol and low density lipoprotein levels compared with control group while it lowers plasma alkaline phosphatase activities and urea (p< 0.05) compared with untreated group. BFBC (400 mg/kg) elevated total protein levels in the pancreas and heart by 103% and 7% compared with the untreated rats. Treatment of diabetic rats with BFBC elevated the body weights by 21% (20 and 200 mg/kg) and 36% (400 mg/kg) respectively. BFBC when administered did not significantly alter hematological, electrolytes and antioxidant enzyme activities in all rats. Histological assessments showed that sections of the pancreas, liver, kidney and heart from BFBC treated animals had reduced tissue damage compared with the untreated groups. Fourteen (14) bioactive compounds highest in oleic, stearic, 2-methyl-pyrrolidine-2-carboxylic, n-hexadecanoic, and 13-docosenoic acids were present in BFBC given Gas-Chromatography/Mass-Spectrometry analysis.
The diabetic animal model was able to present the natural history of the disease in human T2D. Also, BFBC doses used in this study demonstrate potentials against in vitro and in vivo oxidative stress, hyperinsulinaemia, dyslipidemia as well as declension in β-cell function in T2D rat experiment. Further, application of some derivatives of BFBC in the treatment of problems associated with T2D may be useful.
Keywords: Buchholzia coriacea, Streptozotocin, Fructose, Type 2 Diabetes, Metformin, Glibenclamide.
You either get what you want or your money back. T&C Apply
You can find more project topics easily, just search
-
SIMILAR BIOCHEMISTRY FINAL YEAR PROJECT RESEARCH TOPICS
-
1. DETERMINATION OF PHYTIC ACID CONTENT, OXALATE CONTENT, TANNIN CONTENT AND CYANIDE CONTENT IN TEN EDIBLE SEEDS
» ABSTRACT Antrinutitional factors re compounds which reduce the nutrient utilization or food intake of plant or plant products (seeds) used as human fo...Continue Reading »Item Type & Format: Project Material - Ms Word | 63 pages | Instant Download | Chapter 1-5 | BIOCHEMISTRY DEPARTMENT
-
2. PHYTOCHEMICAL SCREENING OF ETHANOL LEAVES EXTRACT OF Jatropha tanjorensis AND ITS EFFECTS ON THE LIVER FUNCTION OF MALE ALBINO WISTAR RATS
» CHAPTER ONE INTRODUCTION 1.1 BACKGROUND INFORMATION Plants have been used sinced the medieval times for medicinal purposes for the treatment of all ki...Continue Reading »Item Type & Format: Project Material - Ms Word | 71 pages | Instant Download | Chapter 1-5 | BIOCHEMISTRY DEPARTMENT
-
3. COMPARATIVE QUANTITATIVE PHYTOCHEMICAL ANALYSIS OF ETHANOL EXTRACT OF Gongronema latifolium AND Allium sativum
» TABLE OF CONTENTS Cover page Title page Certification i Dedication ii Acknowledgement iii Abstract iv Table of content V List of tables vi CHAPTER ONE...Continue Reading »Item Type & Format: Project Material - Ms Word | 44 pages | Instant Download | Chapter 1-5 | BIOCHEMISTRY DEPARTMENT
-
4. CARDIO PROTECTIVE ACTIVITIES OF N-HEXANE EXTRACT OF DESMODIUM VELUTINUM STEM ON ALBINO WISTER RAT
» ABSTRACT This study evaluates the effect of the cardio protective N hexane extract of Desmodium stem velutinum on wister albino rat. The rats used in ...Continue Reading »Item Type & Format: Project Material - Ms Word | 50 pages | Instant Download | Chapter 1-5 | BIOCHEMISTRY DEPARTMENT
-
5. DIFFERENTIAL EXPRESSION LEVELS OF GLUTATHIONE-S-TRANSFERASE (OvGST1) GENE IN THE HOST (HUMAN) AND VECTOR Simulium damnosum
» Abstract Onchocerciasis, a neglected tropical disease (NTD), also known as river blindness, the world‟s second leading cause of preventable blindnes...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | BIOCHEMISTRY DEPARTMENT
-
6. AN ASSESSMENT OF TEACHERS’ KNOWLEDGE OF TEST CONSTRUCTION PROCEDURE IN CHEMISTRY OBJECTIVE TEST IN SENIOR SECONDARY SCHOOLS
» CHAPTER ONE INTRODUCTION Background of Study Testing has always been an integral part of the educational system since its inception. The concept (test...Continue Reading »Item Type & Format: Project Material - Ms Word | 90 pages | Instant Download | Chapter 1-5 | BIOCHEMISTRY DEPARTMENT
-
7. EFFECTS OF ETHANOLIC ROOT EXTRACT OF Jatropha tanjorensis ON LIPID PROFILE OF FEMALE ALBINO WISTAR RAT
» ABSTRACT This research work was carried out to determine the effects of ethanolic root extract of Jatropha tanjorensis on lipid profile of female albi...Continue Reading »Item Type & Format: Project Material - Ms Word | 62 pages | Instant Download | Chapter 1-5 | BIOCHEMISTRY DEPARTMENT
-
8. AMELIORATING ROLE OF N.P.K. FERTILIZER ON THE TOXIC EFFECTS OF Ni ON (SORGHUM) ROOT ANTIOXIDANT ENZYMES
» ABSTRACT This study investigated the activities of superoxide dismutase (SOD), catalase (CAT), glutothione peroxidase (GP) and the level of malondiald...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | BIOCHEMISTRY DEPARTMENT
-
9. PROXIMATE AND PHYSICOCHEMICAL ANALYSIS OF FISH FLOUR OBTAINED FROM THREE FISH SPECIES
» ABSTRACT Work on production proximate and physicochemical analyses of fish flour produced from Tilapia, cat and stock fishes was investigated. The fis...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | BIOCHEMISTRY DEPARTMENT
-
10. PHYTOCHEMICAL ANALYSIS CONSTITUENT OF ABELMOSCHUS ESCULENTUS OF ETHYLE ACAELETE EXTRACTION
» CHAPTER ONE 1.0 INTRODUCTION 1.1 BACKGROUND OF STUDY The presence of chemical constituents in plants is important for consuming as nutrition and treat...Continue Reading »Item Type & Format: Project Material - Ms Word | 55 pages | Instant Download | Chapter 1-5 | BIOCHEMISTRY DEPARTMENT