Get the complete project »
- The Complete Research Material is averagely 116 pages long and it is in Ms Word Format, it has 1-5 Chapters.
- Major Attributes are Abstract, All Chapters, Figures, Appendix, References.
- Study Level: MTech, MSc or PhD.
- Full Access Fee: ₦10,000
Type 2 diabetes (T2D) occurs when there is an advanced determent in insulin action (insulin resistance, IR), which proceeds toβ-cell dysfunction. This present study assessed the modulatory effects of Buchholzia coriacea (B. coriacea) seeds extract in high fructose-fed, streptozotocin-induced T2D in male Wistar rats.
Methanolic extract (MEBC), hexane fraction (HFBC), ethyl acetate fraction (EFBC) and n-butanol fraction of BC (BFBC) were prepared using 70% methanol and successive solvent partitioning method respectively. Antioxidant activities of 1-1-diphenyl 2-picryl hydrazyl (DPPH), nitric oxide radical scavenging assay (NOSA) and hydroxyl radical scavenging activities (HRSA) were assessed as well as α-amylase inhibition in vitro. High fructose (20%, p.o) (2 weeks) followed by streptozotocin (STZ) (40 mg/kg, i.p.) (FRU + STZ) (day 14) administered to achieve T2D in vivo. Control normal and diabetic untreated (FRU + STZ) rats were administered carboxymethyl cellulose (CMC) (1 ml/kg, p.o). Diabetic treated rats received BFBC (20, 200, 400 mg/kg, p.o), metformin (7.14 mg/kg, p.o) and glibenclamide (0.07 mg/kg, p.o) respectively.
BFBC had the highest percentage yield, DPPH and α-amylase inhibition activities, although, EFBC had a better inhibitory activities on HRSA and NOSA respectively. Also, untreated diabetic rats showed increase (p< 0.05 - 0.001) in blood glucose levels (BGLs), insulin (6 folds) and lipid peroxidation (LPO) levels in pancreas when compared with normal group. BFBC (20, 200, 400 mg/kg) showed decrease (p< 0.05) in BGLs in a time dependent manner in the BFBC treated animals.Similarly, BFBC produced a dose dependent decrease in serum insulin levels by 51% (20 mg/kg), 54% (200 mg/kg) and 70% (400 mg/kg) respectively.These effects were also comparable to metformin and glibenclamide. BFBC treatments elevated (p> 0.05) high density lipoprotein, but decreased (p> 0.05) triglycerides, total cholesterol and low density lipoprotein levels compared with control group while it lowers plasma alkaline phosphatase activities and urea (p< 0.05) compared with untreated group. BFBC (400 mg/kg) elevated total protein levels in the pancreas and heart by 103% and 7% compared with the untreated rats. Treatment of diabetic rats with BFBC elevated the body weights by 21% (20 and 200 mg/kg) and 36% (400 mg/kg) respectively. BFBC when administered did not significantly alter hematological, electrolytes and antioxidant enzyme activities in all rats. Histological assessments showed that sections of the pancreas, liver, kidney and heart from BFBC treated animals had reduced tissue damage compared with the untreated groups. Fourteen (14) bioactive compounds highest in oleic, stearic, 2-methyl-pyrrolidine-2-carboxylic, n-hexadecanoic, and 13-docosenoic acids were present in BFBC given Gas-Chromatography/Mass-Spectrometry analysis.
The diabetic animal model was able to present the natural history of the disease in human T2D. Also, BFBC doses used in this study demonstrate potentials against in vitro and in vivo oxidative stress, hyperinsulinaemia, dyslipidemia as well as declension in β-cell function in T2D rat experiment. Further, application of some derivatives of BFBC in the treatment of problems associated with T2D may be useful.
Keywords: Buchholzia coriacea, Streptozotocin, Fructose, Type 2 Diabetes, Metformin, Glibenclamide.
You either get what you want or your money back. T&C Apply
You can find more project topics easily, just search
SIMILAR BIOCHEMISTRY FINAL YEAR PROJECT RESEARCH TOPICS
» ABSTRACT The pulp of Detarium microcarpum fruit was obtained and was analyzed for the proximate, mineral and anti nutrient composition using standard ...Continue Reading »
» CHAPTER ONE INTRODUCTION 1.1 Introduction Gout was described by Hippocrates as “the disease of kings” due to its association with rich diet. (Fala...Continue Reading »
» TABLE OF CONTENTS Title Page Cover Page Certification Dedication Acknowledgement Table of Contents Abstract List of Tables List of Figures CHAPTER ONE...Continue Reading »
4. COMPARATIVE STUDY OF THE ADSORPTION CAPACITIES OF CHEMICALLY AND MICROWAVE SYNTHESIZED ACTIVATED CARBON» CHAPTER ONE INTRODUCTION 1.1 BACKGROUND OF STUDY Activated carbon, also widely known as activated charcoal or activated coal is a form of carbon which...Continue Reading »
» CHAPTER ONE INTPDUCTION Background of the Study The coconut (Cocos nucifera L.) is a tropical plant which belongs to the order Arecales and family Are...Continue Reading »
» ABSTRACT This work evaluates the antioxidant potential of Monodora myristica (African nutmeg). Monodora myristica extract was obtained by solvent extr...Continue Reading »
» CHAPTER ONE 1.0 INTRODUCTION 1.1 BACKGROUND OF STUDY Most of traditional medicines are of plant origins. Nature has gifted us with so many medic...Continue Reading »
8. BRAIN ANTIOXIDANT ACTIVITIES OF SIX ARTEMISININ-BASED COMBINATION THERAPIES (ACTs) IN EXPERIMENTAL MALARIA MODEL» ABSTRACT Malaria parasite has remained a menace to human immune system as it usually subjects its host to oxidative stress which in turn has an effect...Continue Reading »
9. INVESTIGATION OF THE PANCREATIC EFFECT OF BUCCHOLZIA CORICACEA FORMULATED DIET IN SUCROSE FED PREGNANT RATS AND THEIR OFFSPRING» ABSTRACT Several plants have been used in the treatment of various disorders without scientific basis. The present study examine the pancreatic effect...Continue Reading »
» ABSTRACT The research focus on compensation policy as means of attracting and retaining employee, it present a conceptual and theoretical study of the...Continue Reading »