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Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories. In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method. Different concentrations of bleach (1%, 2%, 3%, 4% and 5%) were used on both organisms. Also (50%, 60%, 70%, 85% and 95%) of ethanol as well as (5%, 10%, 20%, 25%, and 30%) Phenolics were used. Diffrences in concentrations tested was because, the original concentrations of the disinfectants differs. After 24 hours of incubation at 370C, the results showed that all the disinfectants inhibited the growth of the test organism in their concentrated forms. The diameter of zone of inhibitions were measured around each well by using a ruler in millimeters, using different concentrations, their efficacies varied. The results showed that 30% Phenolics had the best efficiency against both test organisms and 5% bleach had a better effect on Staphylococcus aureus than Pseudomonas aeruginosa, while ethanol showed least sensitivity. 70% concentration gave the highest effect on Staphylococcus aureus as compared withPseudomonas aeruginosa.
Microorganisms are minute living things that individually are too small to be seen with the unaided eyes (Tortora et al, 2007). Though only a minority of microorganisms are pathogenic (disease producing), practical knowledge of microbes is necessary for medicine and related health sciences. For example hospital workers must be able to protect patients from common microbes that are normally harmless but pose a threat to the sick and injured. Thousands of people died in devastating epidemics; the cause of which was not understood. Entire families died because vaccination and antibiotics were not available to fight infection (Johnson and Case, 1995). This leads to scientific control of microbial growth. This began only about 100 years ago. It was Pasteur’s work on microorganism that led scientists to believe that microbes were a possible cause of diseases and need to be eliminated or destroyed. Some examples off these microbes are; Bacteria, fungi, viruses and protozoa etc (Tortora et al, 2007).
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SIMILAR MICROBIOLOGY FINAL YEAR PROJECT RESEARCH TOPICS
1. ANTITRYPANOSOMAL ACTIVITIES OF ACACIA NILOTICA AND KHAYA SENEGALENSIS AND THE HAEMATOLOGICAL PROFILE OF TRYPANOSOMA BRUCEI BRUCEI INFECTED WISTAR RATS» ABSTRACT This study was aimed at investigating antitrypanosomal activities and haematological profile of crude extract and fractions of the stem bark ...Continue Reading »
2. EFFICACY OF ARTESUNATE IN THE TREATMENT OF URINARY SCHISTOSOMIASIS AT UMUIKWU- ANAM COMMUNITY IN ANAMBRA WEST LOCAL GOVERNMENT AREA OF ANAMBRA STATE, ...» ABSTRACT A study on the efficacy of artesunate in the treatment of urinary schistosomiasis at Umuikwu Anam community, Anambra West Local Government ar...Continue Reading »
» ABSTRACT The samples of male and female body surfaces in Osun State Polytechnic Iree were collected for culturing analysis. Two fungi were isolated an...Continue Reading »
4. DESIGN AND IMPLEMENTATION OF AN EXPERT SYSTEM ON THYPHOID AND MALARIA DIAGNOSIS (A case study of Park Lane Hospital Enugu)» CHAPTER ONE INTRODUCTION 1.1 Background of the Study An expert system is a software system that attempts to reproduce the performance of one or more h...Continue Reading »
» ABSTRACT The study surveyed the prevalence of malaria as well as management practices adpted in Ndiegoro community, Aba South L.G.A., Abia state betwe...Continue Reading »
6. MOSQUITO SURVEY THROUGH LARVAL SAMPLING AND OVITRAPPING IN PARTS OF ASABA, DELTA STATE, SOUTHWESTERN NIGERIA.» ABSTRACT A survey of the mosquito fauna of Asaba, the Delta State capital of Southwestern Nigeria was undertaken between January and March 2009. The m...Continue Reading »
7. ANTIMICROBIAL ACTIVITY OF VINEGAR, HONEY, BLACK PEPPER, GINGER AND MUSHROOM EXTRACT ON WOUND ISOLATE» Abstract Suspensions of vinegar, honey, black pepper and ginger were examined for antibacterial activity against Salmonella sp., Pseudomonas sp. and E...Continue Reading »
8. INTESTINAL PARASITES AMONG UNITY PRIMARY SCHOOL PUPILS, IN ORAIFITE, EKWUSIGO L.G.A., ANAMBRA STATE, SOUTHEASTERN NIGERIA.» ABSTRACT A study was conducted to determine the prevalence of intestinal parasites among pupils in Unity primary school in Oraifite, Ekwusigo Local Go...Continue Reading »
9. MULTIDRUG RESISTANCE PROFILE AND METALLO-BETA-LACTAMASE PRODUCTION IN Pseudomonas aeruginosa ISOLATED FROM THE NASAL CAVITY OF CATTLE FROM KARA MARKET...» ABSTRACT Pseudomonas aeruginosa is an ubiquitous, Gram negative, rod shaped, monoflagellated bacterium, capable of causing serious infections in immun...Continue Reading »
10. BIOREMEDIATION OF EFFLUENT FROM KADUNA REFINERYAND PETROCHEMICAL COMPANY USING CLADOSPORIUM SPECIES» ABSTRACT This study investigated the potential bioremediation ability of Cladosporium species in hydrocarbon contaminated wastewater. Hydrocarbon cont...Continue Reading »