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ABSTRACT
Dermatophytosis is a common fungal infection of the skin in humans and animals. This
study evaluates anti-fungal activity of Chlorophytum laxum (R Br) extract on fungi
isolates associated with tinea capitis. The whole plant was extracted and subjected to
phytochemical screening and the fractions were analyzed using gas- chromatography and
mass spectrometry. The bioassay was done using food poisoning technique method.
Trichophyton spp and Microsporum spp were the fungal isolated and identified. The
crude extract of Chlorophytum laxum at 50mg/ml showed the highest zone of inhibition
on the isolates: Microsporum spp (30.00 ± 6.1), trichophyton spp (37.3 ± 1.2), and
trichophyton spp (60.3 ± 0.9) respectively significantly different (P < 0.05). Gas-
chromatography/Mass-Spectrometry analysis identified Dimethyl sulfone, 4-
Methylvaleric acid, 1-Propanol and 5H- Tetrazol-5-amine as bioactive compounds that
have antifungal properties in the fractions. The data obtained were subjected to analysis
of variance at p<0.05 using SPSS version 20 and means were separated using least
significant difference (LSD) This study therefore showed that, the effectiveness of
C.laxum inhibit Microsporum spp and Trichophyton spp which were found to causes
tinea capitis infection from scalp scrapings among primary school pupils in Zaria and
suggests the scientific reason for the traditional use of the plant in treatment of fungal
infection.
CHAPTER ONE
INTRODUCTION
1.1 Background of Study
Chlorophytum laxum R. Br. Family (Liliaceae) is a traditional endangered perennial
herbaceous medicinal plants (Patil and Kumar, 2011; Chakraborthy et al.,
2014).Medicinal plants have been identified and used throughout human history. Plants
have the ability to synthesize a wide variety of chemical compounds that are used to
perform important biological functions and defense against infections caused by insects,
fungi and herbivorous mammals. Many of the herbs and spices used by humans to season
food give useful m
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