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ABSTRACT
Dairy wastewater is increasingly becoming an environmental concern. There is a widespread interest
in the use of beta-galactosidase (EC 3.2.1.23) to hydrolyze lactose in milk and dairy products which
aid in improving their functional and digestive properties. An attempt was made to isolate fungi from
dairy effluent using standard culture techniques. Physicochemical characteristics and proximate
composition of the dairy effluent was analyzed. The fungi isolated were subsequently screened using
Ortho-nitrophenol-beta-D-galactopyranoside (ONPG) discs method for potential to produce beta-
galactosidase. The isolate showing beta-galactosidase activity was selected and used to produce
beta-galactosidase under submerged fermentation using the dairy effluent as a substrate. A total of
nine (9) fungi were isolated from the dairy effluent belonging to the Genera Aspergillus, Rhizopus,
Rhizomucor and Penicillium with Aspergillus having the highest frequency of occurrence (67%).
Physicochemical analyses of the raw dairy effluent revealed the effluent had a pH of 6.8 (±0.20),
electrical conductivity of 645µs/cm (±1.00) and total dissolved solids of 324mg/L (±2.00). The
dissolved oxygen and biochemical oxygen demand were 200mg/L (±1.00) and 100mg/L (±1.70)
respectively while the nitrates, phosphates and sulfates were 1.4x10-3% (±1.0x 10-4), 54.94mg/L
(±0.79) and 13.66mg/L (±0.61) respectively. The proximate composition of the dairy effluent
revealed ash, lipid, protein and carbohydrate contents were 0.15% (±0.01), 5.85% (±0.02), 0.88%
(±0.01) and 13.13% (±0.00) respectively. Only Rhizopus species showed beta-galactosidase activity
and was used to produce extracellular beta-galactosidase. A maximum beta-galactosidase activity of
4.65U/mL (±0.02) was obtained with the selected Rhizopus species. It was concluded that Rhizopus
species could be a potential fungal strain for beta-galactosidase production using the dairy effluent as
substrate.
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SIMILAR MICROBIOLOGY FINAL YEAR PROJECT RESEARCH TOPICS
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1. ANTIBACTERIAL ACTIVITY OF HONEY ON Staphylococcus aureusEscherichia coli and Streptococcus pyogen ISOLATED FROM WOUND
» ABSTRACT Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus,...Continue Reading »Item Type & Format: Project Material - Ms Word | 66 pages |
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2. DETECTION OF ANTIBIOTIC RESISTANCE AND VIRULENT GENES IN ENTEROCOCCI ISOLATED FROM WATER SAMPLES IN ZARIA, KADUNA STATE, NIGERIA.
» ABSTRACT The present study was conducted to isolate, identify and analyse the distribution of Enterococci,carry out antibiotic susceptibility test and...Continue Reading »Item Type & Format: Project Material - Ms Word | 5200 pages |
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3. ISOLATION AND IDENTIFICATION OF MICROORGANISM IN STREAM WATER [ATOLAGBE] IREE TOWN
» ABSTRACT The stream water was examined for microorganism present in it. The isolated and identified bacteria are Bacillus cereus and Staphylococcus au...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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4. BACTERIA ASSOCIATED WITH URINARY TRACT INFECTION IN PREGNANT WOMEN AND THEIR ANTIBIOTIC SUSCEPTIBILITY
» ABSTRACTUrine Sample from pregnant women were analysed for bacteriuria. The result of irinalysis reveals the appearance –yellow and Cloudy, pH 6.0, ...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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5. FUNGAL COLONIZATION ON HUMAN BODY SURFACES
» ABSTRACT The samples of male and female body surfaces in Osun State Polytechnic Iree were collected for culturing analysis. Two fungi were isolated an...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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6. COMPARATIVE TOXICITY OF CRUDE OIL AND KEROSENE ON THE GROWTH OF NITRIFYING BACTERIA ISOLATED FROM SOIL
» ABSTRACTCrude oil and kerosene have useful applications in the society and are, therefore, used as sources of income and energy. However, the spills a...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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7. ANTIBACTERIAL ACTIVITIES OF Carica papaya SEED AGAINST ENTERIC ORGANISMS
» ABSTRACT Lots of research has been conducted on Carica papaya and its antibacterial efficacy on bacterial pathogens and this led to this study on anti...Continue Reading »Item Type & Format: Project Material - Ms Word | 37 pages |
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8. MYCOFLORA OF STORED LOCUS BEANS (Parkia biglobosa ) PURCHASED FROM USELU MARKET
» ABSTRACT Stored locust bean samples were purchased from Uselu market and analysed for their fungal load and physicochemical properties such as moistur...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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9. PARASITOLOGICAL EXAMINATION OF SOME READY TO EAT FRUITS SOLD AT OYE EMENE ENUGU STATE NIGERIA
» ABSTRACT Some locally available fruits (garden egg, orange, banana, guava, avocado, pawpaw and pineapple).sold in Oye Emene Enugu, Enugu State was exa...Continue Reading »Item Type & Format: Project Material - Ms Word | 42 pages |
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10. COMPARATIVE STUDY OF GLUTAMIC ACID PRODUCTION BY WILD-TYPE AND MUTANT STRAINS OF CORYNEBACTERIUM GLUTAMICUM
» ABSTRACT Several different lignocellulosic biomass of agricultural origin hold remarkable potential for conversion into commodity products presenting ...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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