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ABSTRACT
Dairy wastewater is increasingly becoming an environmental concern. There is a widespread interest
in the use of beta-galactosidase (EC 3.2.1.23) to hydrolyze lactose in milk and dairy products which
aid in improving their functional and digestive properties. An attempt was made to isolate fungi from
dairy effluent using standard culture techniques. Physicochemical characteristics and proximate
composition of the dairy effluent was analyzed. The fungi isolated were subsequently screened using
Ortho-nitrophenol-beta-D-galactopyranoside (ONPG) discs method for potential to produce beta-
galactosidase. The isolate showing beta-galactosidase activity was selected and used to produce
beta-galactosidase under submerged fermentation using the dairy effluent as a substrate. A total of
nine (9) fungi were isolated from the dairy effluent belonging to the Genera Aspergillus, Rhizopus,
Rhizomucor and Penicillium with Aspergillus having the highest frequency of occurrence (67%).
Physicochemical analyses of the raw dairy effluent revealed the effluent had a pH of 6.8 (±0.20),
electrical conductivity of 645µs/cm (±1.00) and total dissolved solids of 324mg/L (±2.00). The
dissolved oxygen and biochemical oxygen demand were 200mg/L (±1.00) and 100mg/L (±1.70)
respectively while the nitrates, phosphates and sulfates were 1.4x10-3% (±1.0x 10-4), 54.94mg/L
(±0.79) and 13.66mg/L (±0.61) respectively. The proximate composition of the dairy effluent
revealed ash, lipid, protein and carbohydrate contents were 0.15% (±0.01), 5.85% (±0.02), 0.88%
(±0.01) and 13.13% (±0.00) respectively. Only Rhizopus species showed beta-galactosidase activity
and was used to produce extracellular beta-galactosidase. A maximum beta-galactosidase activity of
4.65U/mL (±0.02) was obtained with the selected Rhizopus species. It was concluded that Rhizopus
species could be a potential fungal strain for beta-galactosidase production using the dairy effluent as
substrate.
CHAPTER ONE
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SIMILAR MICROBIOLOGY FINAL YEAR PROJECT RESEARCH TOPICS
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1. PHYTOCHEMICAL AND ANTIMICROBIAL ANALYSIS OF MYSTLETOE LEAVES
» CHAPTER ONE 1.0 INTRODUCTION The use of the leaves, stem and bark of the plants for medicinal purposes is an age long practice. Also in the modern tim...Continue Reading »Item Type & Format: Project Material - Ms Word | 55 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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2. MULTIDRUG RESISTANCE PROFILE AND METALLO-BETA-LACTAMASE PRODUCTION IN Pseudomonas aeruginosa ISOLATED FROM THE NASAL CAVITY OF CATTLE FROM KARA MARKET...
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3. DETERMINING THE POTENTIALS OF LACTIC ACID BACTERIA ISOLATED FROM NONO FOR USE AS STARTER CULTURES IN YOGHURT PRODUCTION
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4. COMPARATIVE STUDY OF DISINFECTANT EFFICIENCY OF ETHANOL, BLEACH AND PHENOLICS AGAINST Pseudomonas aeruginosa and Staphylococcus aureus
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5. INVESTIGATION OF ANAEROBIC PROCESSES IN SEPTIC TANK AS A WASTEWATER TREATMENT OPTION
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6. DETERMINATION OF THE MICROBIAL LOAD ON SPOILT EGUSI
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7. COMPARATIVE STUDY OF ANTIBACTERIAL ACTIVITY OF TWO SELECTED MEDICATED SOAP AND ONE LOCAL BLACK SOAP ON STAPHYLOCOCCUS AUREUS FROM WOUND INFECTION
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8. DETECTION OF HEPATITIS C AND HEPATITIS B VIRUS INFECTION AMONG PRISON INMATES AND PSYCHIATRIC PATIENTS IN KADUNA METROPOLIS, NIGERIA
» ABSTRACT There is high risk of contracting hepatitis B and C among individuals with psychotic disorders due to lifestyle factors and prisoners globall...Continue Reading »Item Type & Format: Project Material - Ms Word | 100 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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9. ANALYSIS OF BUSH PEAR AND ITS OIL
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10. PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF Acalypha wilkesiana EXTRACT AGAINST CLINICAL ISOLATES OF Candida albicans
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