This study investigated the job satisfaction of professional nurses in public and private health sectors in Anambra State. A survey design was employed and a study population of 5903 comprising all professional nurses in private and public hospitals was used. Proportionate stratified random sampling technique was used in selecting a sample of 375 nurses for the study. Instrument for data collection was a structured questionnaire. Data collected were analyzed using mean and standard deviation statistical tool to answer the four research questions and t-test statistical tool was used to test the four hypotheses. Findings showed that nurses in public hospitals were satisfied from job security unlike nurses in private hospitals. Nurses in public and private hospitals were satisfied from job control/ responsibilities. Also, it was found that opportunity for advancement guarantees job satisfaction to nurses in public and private hospitals. Based on the findings, it was recommended that hospital management should create a work environment that is free from dissatisfiers in order that nurses would carry out their duties effectively towards the actualization of organization’s goal. Few relevant areas that the present study did not cover were suggested for further investigation.