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Crude oil and kerosene have useful applications in the society and are, therefore, used as sources of income and energy. However, the spills arising from crude oil and kerosene in our environment are becoming a visible problem in our ecosystem. Crude oil and kerosene pollute our farmlands and other arable lands and may negatively affect the growth and activities of nitrifying bacteria responsible for nitrification process, leading to loss of soil fertility. Considering the important roles played by nitrifying bacteria in soil fertility in our ecosystem, the assessment of the response of the nitrifying bacteria to pollution stress exerted by crude oil and kerosene on them was investigated. Two genera of nitrifying bacteria were isolated from the soil and were identified asNitrosomonas sp and Nitrobacter sp based on their morphological, biochemical and physiological characteristics. Nitrosomonas sp was isolated using Winogradsky medium for nitrification phase I while Nitrobacter sp was isolated using Winogradsky medium for nitrification phase II. A range of crude oil and kerosene concentrations (in triplicates) were prepared and introduced into sterile Winogradsky salt broth to arrive at final concentrations of 0.5%, 1%, 2%, 5% and 10% (v/v) respectively. The flasks were thereafter inoculated with standardized pure cultures of Nitrosomonas sp and Nitrobacter sp using a uniform inoculum size of 1ml of 1.5 x106 cells for Nitrosomonas sp and 1.2 x106 cells for Nitrobacter sp and incubated for 120 hours. The result of the toxicity test carried out on these isolates showed that the sensitivity of each test organism was a function of both the contact time and concentrations of crude oil and kerosene. The effect of different concentrations of crude oil and kerosene on the oxidation of nitrite to nitrate showed that oxidation of nitrite to nitrate was hindered at higher concentrations of the two pollutants, but, at lower concentrations, crude oil and kerosene did not hinder oxidation of nitrite to nitrate. Information generated from various tests can be of use in the management of pollution for the purpose of prediction of environmental effects of crude oil and kerosene, comparison of toxicants or organisms or test conditions and regulation of crude oil and kerosene spills. For instance this study will help us to provide information for regulatory purposes that a soil contaminated with certain percentage of crude oil or kerosene should not be used for agricultural purposes or practices.
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SIMILAR MICROBIOLOGY FINAL YEAR PROJECT RESEARCH TOPICS
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1. CODIGESTION OF ZEA MAYS AND CITRULLUS COLOCYNTHIS WASTES FOR BIOGAS GENERATION
» TABLE OF CONTENTS Pages Title Page i Certification ii Dedication iii Acknowledgements iv Table of Contents v List of Tables x List of Figures xi Abstr...Continue Reading »Item Type & Format: Project Material - Ms Word | 70 pages |
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2. EFFICACY OF ARTESUNATE IN THE TREATMENT OF URINARY SCHISTOSOMIASIS AT UMUIKWU- ANAM COMMUNITY IN ANAMBRA WEST LOCAL GOVERNMENT AREA OF ANAMBRA STATE, ...
» ABSTRACT A study on the efficacy of artesunate in the treatment of urinary schistosomiasis at Umuikwu Anam community, Anambra West Local Government ar...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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3. A SURVEY OF THE ANTIBACTERIAL ACTIVITY OF THYMUS VULGARIS AND MYRISTICA FRAGRANS ON STREPTOCOCUSS PNEUMONIAE AND PSEUDOMONAS AERUGINOSA
» ABSTRACT The aqueous and ethanolic extracts of the leaves of Thymus vulgaris (thyme) and the seeds of Myristica fragrans (nutmeg) were screened for th...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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4. MOSQUITO SURVEY THROUGH LARVAL SAMPLING AND OVITRAPPING IN PARTS OF ASABA, DELTA STATE, SOUTHWESTERN NIGERIA.
» ABSTRACT A survey of the mosquito fauna of Asaba, the Delta State capital of Southwestern Nigeria was undertaken between January and March 2009. The m...Continue Reading »Item Type & Format: Project Material - Ms Word | 109 pages |
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5. ISOLATION AND IDENTIFICATION OF BACTERIA FROM FOOD VENDORS AND SOME VEGETABLE AVAILABLE AT OGBETE MARKET ENUGU.
» ABSTRACT Safety of food is a basic requirement of food quality. A total of 25 street food samples (Jollof rice, egwusi soup, ugu, water leaf and green...Continue Reading »Item Type & Format: Project Material - Ms Word | 66 pages |
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6. RELATIONSHIP BETWEEN WEIGHT AND BLOOD PRESSURE OF PATIENTS WITH HIGH BLOOD PRESSURE
» CHAPTER ONE1.0 Introduction1.1 Background of the Study The number of people with high blood pressure is in the increase and research has shown that th...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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7. MYCOFLORA OF STORED LOCUS BEANS (Parkia biglobosa ) PURCHASED FROM USELU MARKET
» ABSTRACT Stored locust bean samples were purchased from Uselu market and analysed for their fungal load and physicochemical properties such as moistur...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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8. EVALUATION OF THE EFFECTS OF ANTHROPOENIC ACTIVITIES AND DOMESTIC WASTE ON SURFACE WATER QUALITY (A CASE STUDY OF RIVER NIGER IN KATCHA L.G.A, NIGER)...
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9. MICROBIOLOGICAL ASSESSMENT OF INDOOR AND OUTDOOR AIR OF FAITH MEDIPLEX HOSPITAL, BENIN CITY
» ABSTRACT This study was aimed at investigating the microbial load and the quality of indoor air Faith Mediplex Centre, Benin City, to ascertain their ...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages |
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10. PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF Acalypha wilkesiana EXTRACT AGAINST CLINICAL ISOLATES OF Candida albicans
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