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Crude oil and kerosene have useful applications in the society and are, therefore, used as sources of income and energy. However, the spills arising from crude oil and kerosene in our environment are becoming a visible problem in our ecosystem. Crude oil and kerosene pollute our farmlands and other arable lands and may negatively affect the growth and activities of nitrifying bacteria responsible for nitrification process, leading to loss of soil fertility. Considering the important roles played by nitrifying bacteria in soil fertility in our ecosystem, the assessment of the response of the nitrifying bacteria to pollution stress exerted by crude oil and kerosene on them was investigated. Two genera of nitrifying bacteria were isolated from the soil and were identified asNitrosomonas sp and Nitrobacter sp based on their morphological, biochemical and physiological characteristics. Nitrosomonas sp was isolated using Winogradsky medium for nitrification phase I while Nitrobacter sp was isolated using Winogradsky medium for nitrification phase II. A range of crude oil and kerosene concentrations (in triplicates) were prepared and introduced into sterile Winogradsky salt broth to arrive at final concentrations of 0.5%, 1%, 2%, 5% and 10% (v/v) respectively. The flasks were thereafter inoculated with standardized pure cultures of Nitrosomonas sp and Nitrobacter sp using a uniform inoculum size of 1ml of 1.5 x106 cells for Nitrosomonas sp and 1.2 x106 cells for Nitrobacter sp and incubated for 120 hours. The result of the toxicity test carried out on these isolates showed that the sensitivity of each test organism was a function of both the contact time and concentrations of crude oil and kerosene. The effect of different concentrations of crude oil and kerosene on the oxidation of nitrite to nitrate showed that oxidation of nitrite to nitrate was hindered at higher concentrations of the two pollutants, but, at lower concentrations, crude oil and kerosene did not hinder oxidation of nitrite to nitrate. Information generated from various tests can be of use in the management of pollution for the purpose of prediction of environmental effects of crude oil and kerosene, comparison of toxicants or organisms or test conditions and regulation of crude oil and kerosene spills. For instance this study will help us to provide information for regulatory purposes that a soil contaminated with certain percentage of crude oil or kerosene should not be used for agricultural purposes or practices.
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SIMILAR MICROBIOLOGY FINAL YEAR PROJECT RESEARCH TOPICS
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1. DETERMINATION OF THE MICROBIAL LOAD ON SPOILT EGUSI
» ABSTRACTFruit juices are very nutritive, invigorating and non alcoholic beverage, which is very well liked throughout the world. Juice may be squeezed...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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2. ANTIBIOGRAM AND MICROBIAL CARRIAGE OF CAMPUS SHUTTLE DOOR HANDLES IN FEDERAL UNIVERSITY OF TECHNOLOGY, AKURE.
» ABSTRACT The transmission of infection via fomites constitutes a major threat to public health especially in the developing countries. This study was ...Continue Reading »Item Type & Format: Project Material - Ms Word | 72 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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3. COMPARATIVE ANALYSIS OF MICROBIAL LOAD OF THE ENUGU MAIN WATER PRODUCTION AND WATER AVAILABLE TO IMT CAMPUS II HOSTEL
» CHAPTER ONE INTRODUCTION Water is a liquid mineral. It consist of two atoms of hydrogen combined with one atom of oxygen. Water is a tasteless, odourl...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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4. MICROBIOLOGICAL ANALYSIS OF DRY CASSAVA PEEL USED IN THE PREPARATION OF PIG FEEDS
» Abstract This study was carried out on the microbiological analysis of dry cassava peel used in the preparation of pigs feed. Since the nutritional co...Continue Reading »Item Type & Format: Project Material - Ms Word | 74 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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5. EFFECT OF MORINGA OLEIFERA LEAF EXTRACT ON THE GROWTH AND YIELD OF PEPPER
» ABSTRACT This study was carried out during the two summer seasons of 2014 and 2015 on pepper (Capsicum annuum L.) cv. California Wonder. The goals of ...Continue Reading »Item Type & Format: Project Material - Ms Word | 31 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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6. EFFECT OF DIFFERENT CARBON SOURCES ON THE GROWTH OF ANTIMICROBIAL PRODUCING Bacillus Species IN Citrillus vugaris.
» CHAPTER ONE 1.0 INTRODUCTION Traditional fermented condiments (OGIRI EGUSI) based on vegetable proteins are consumed by different ethnic groups in Nig...Continue Reading »Item Type & Format: Project Material - Ms Word | 75 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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7. ISOLATION IDENTIFICATION AND CHARACTERIZATION OF FUNGI ASSOCIATED WITH THE SPOILAGE OF CORN
» TABLE OF CONTENTSCHAPTER ONE1.0 Introduction 1.1 Statement of the problem 1.2 Aims/Objective of the Study 1.3 Limitation of the Study 1.4 Significance...Continue Reading »Item Type & Format: Project Material - Ms Word | 65 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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8. CODIGESTION OF ZEA MAYS AND CITRULLUS COLOCYNTHIS WASTES FOR BIOGAS GENERATION
» TABLE OF CONTENTS Pages Title Page i Certification ii Dedication iii Acknowledgements iv Table of Contents v List of Tables x List of Figures xi Abstr...Continue Reading »Item Type & Format: Project Material - Ms Word | 70 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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9. ANTIBACTERIAL ACTIVITY OF HONEY ON Staphylococcus aureusEscherichia coli and Streptococcus pyogen ISOLATED FROM WOUND
» ABSTRACT Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus,...Continue Reading »Item Type & Format: Project Material - Ms Word | 66 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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10. ELEMENT OF ANALYSIS ON PTEROCARPUS MILDBEADII
» INTRODUCTION Nature has always been the mother of all treatments, which provides therapies for all illnesses and diseases. Through the process of stud...Continue Reading »Item Type & Format: Project Material - Ms Word | 97 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT