Get the complete project »
- The Complete Research Material is averagely 52 pages long and it is in Ms Word Format, it has 1-5 Chapters.
- Major Attributes are Abstract, All Chapters, Figures, Appendix, References.
- Study Level: BTech, BSc, BEng, BA, HND, ND or NCE.
- Full Access Fee: ₦4,000
The potential effect of methanolic extract of the leaves of Acalypha wilkesiana on the weight of wistar rats was investigated. Calculated amount of methanolic leaf extract of Acalypha Wilkesiana were constituted in distilled water from the stock solution to give doses of 500,500 and 1000 mg/kg body weight of paracetamol, vitamin C and leaf extract respectively and administered to the various groups (A, B, C and D). Prior to the administration of methanolic leaf extract of Acalypha Wilkesiana at every interval of seven days, the body weights of the animals were recorded. The acute administration of the aqueous leaf extract of Acalypha wilkesiana did not result in obvious signs of morphological changes or death of male rats throughout the experimental period. A significant difference (P>0.05) was obtained in the average body weight of animals administered with the extract as compared with the paracetamol treated group. The organ weight also elucidated no significant differences (P<0.05) in the kidney, brain, heart, lung and pancreas except for the liver where there was an increase in the weight of the liver treated with extract as compared to that of the rats treated with paracetamol. From the results obtained it is shown that the extract altered the weight of the liver and is therefore not hepatoprotective, this extract should undergo further investigation before oral administration is recommended.
INTRODUCTION AND LITERATURE REVIEW
1.1 MEDICINAL PLANTS
The use of plants for healing purposes has always been part of human culture and it is getting increasingly popular in Nigeria. Acalypha wilkesiana is one of several medicinal plants used in Nigeria and it has various ethno botanical uses. Acalypha wilkesiana belongs to the family Euphorbiaceae. It is propagated by stem cuttings at any time of the year. Under ideal conditions, it grows as a spreading evergreen shrub with upright branches that tend to originate near the base and can get up to 3.1 m tall with a similar spread. It has leafs (12.7- 20.3 cm long) that are alternate, elliptic to oval, serrate and multi-coloredans small inconspicuous flowers (10.2-20.3 cm) that hangs in catkin-like racemes beneath the foliage
In some parts of southern Nigeria, the use of diuretics in the treatment of hypertension has been traditionally substituted for aqueous leaf extract of Acalypha wilkesiana. Acute changes in body mass over a short time period can frequently be assumed to be due to body water loss or gain; 1 ml of water has a mass of 1 g and therefore changes in body mass can be used to quantify water gain or loss. Over a short time period, no other body component will be lost at such a rate, making this assumption possible (Shirreffs, 2003), thus weight parameters were evaluated and used as makers of hydration status of the male Wistar rats.
1.1.1 ACALYPHA SPECIE
Subtribe: Acalyphinae Genus Acalypha
Acalypha is a plant genus of the family Euphorbiaceae. It is the sole genus of the subtribe Acalyphinae. With 450 to 500 species of shrubs, trees and annuals, the genus is only behind Euphorbia, Croton and Phyllanthus in terms of Malpighiales diversity. The common name is copperleaf, three-seeded mercury or cat's tail (Pax et al, 1924). These plants are mostly tropical or subtropical, with a few representatives in temperate zones. The Americas contain two thirds of the known species, distributed from southern United States to Uruguay and northern Argentina. Several species, such as Acalypha ecuadorica, Acalypha eggersii and Acalypha raivavensis are nearly extinct, and the St. Helena Mountain Bush or "stringwood" Acalypha rubrinervis already is hispida (chenille plant, red-hot cat's tail), cultivated as a houseplant because of its colourful and texturally exciting flowers, has gained the Royal Horticultural Society's Award of Garden Merit. (Grubben et al, 2004), as has Acalypha hispaniolae (Hispaniola cat's tail). Others are grown for their foliage and a number of cultivars have been developed, such as Acalypha wilkesiana 'Obovata Cristata' and Acalypha wilkesiana Acalypha wilkesiana “Hoffmannii'. Acalypha bipartita is eaten as a vegetable in some parts of Africa.
1.1.2 ACALYPHA WILKESIANA
Species: Acalypha wilkesiana
Binomial name: Acalypha wilkesiana
Acalypha wilkesiana is an evergreen shrub. It grows 3 m high and spreads 2 m across. The stem is erect with many branches. The branches have fine hairs. It has a closely arranged crown. The leaves are coppery green with red splashes of colour. This gives them a mottled appearance. The leaves are large and broad with teeth around the edge. They can be 10–20 cm long and 15 cm wide. The leaves are finely hairy. They can be flat or crinkled. The flowers are reddish in spikes at the end of branches. They have separate male and female flowers on the same plant. The male flowers are in long spikes which hang downwards while the female flowers are in short spikes. They do not show up easily as they are often hidden among the leaves. The flower stalks are 10–20 cm long. A tropical and subtropical plant which grows naturally in Vanuatu. It occurs in the Pacific Islands. It prefers light well drained soil. It suits a protected shady position. It is damaged by both drought and frost. It needs a minimum temperature above 10°C. It suits hardiness zones 9-12. Acalypha wilkesiana ointment is used to treat fungal skin diseases (Oyelami et al, 2003) carried out a non-comparative study to evaluate the safety and efficiency of Acalypha wilkesiana ointment using 32 Nigerians with mycological as well as clinical evidence of mycoses. The ointment successfully controlled the mycoses in 73.3% of the affected patients. It was very effective in treating Pityriasis versicolor, Tinea pedia and Candida intetrigo, with 100% cure (Oyelami et al, 2003) concluded that Acalypha wilkesiana ointment can be used to treat superficial mycoses (Akinyemi et al, 2005) evaluated crude extracts from six important medicinal plants, namely: Phylantus discoideus, Ageratum conyzoides, Terminalia avicennioides, Bridella ferruginea, Acalypha wilkesiana and Ocimum gratissimum, to find activity against methicillin resistant Staphylococcus aureus (MRSA. Water and ethanolic extracts of these plants were obtained locally. MRSA strains isolated from patients were used. Both ethanolic and water extracts showed effects on MRSA. Minimum bactericidal concentration (MBC) and minimum inhibition concentration (MIC) of these plants ranged from 30.4-37.0 mcg/ml and 18.2-24.0 mcg/ml respectively. A high MBS value was found in two plants and the other four contained traceable amounts of anthraquinones. This study provided scientific support for the use of Acalypha wilkesiana, T. avicennioides, O. gratissimum and P. discoidens against MRSA based diseases. A. conyzoides and B. ferruginea were unresponsive against the MRSA strains (Oyelami et al, 2003).
Fig 1: structure of paracetamol
Paracetamol (called acetaminophen in the USA) is one of the most commonly used non-narcotic analgesic and antipyretic agents. It has relatively weak anti-inflammatory activity. Paracetamol is reported to be selective inhibitor of Cox 3 (cyclooxygenase). Although some reported evidence show that paracetamol has significant anti- inflammatory action (Granberg et al, 1999). Paracetamol toxicity is one of the most common causes of poisoning worldwide. In the United States and United Kingdom it is the most common cause of acute liver failure. Paracetamol was the fourth most common cause of death following self-poisoning in the United Kingdom in 1989; (karthikeyan et al., 2005), yet it is still one of the most common analgesic and antipyretic drugs often used around the world to treat pains and mild feverish conditions. As far as this is true, it is also one of the major causes of liver damage such as liver necrosis. Traditionally, a number of herbal medicines have been used in ameliorating this problem of hepatotoxicity such as fresh garlic; (moller et al, 2009), methanolic extract of Acalypha wilkesiana; (khashab et al, 2007).Toxic doses of paracetamol cause a serious potentially fatal hepatotoxicity.
1.2.1 MEDICAL USES OF PARACETAMOL
In medicine paracetamol is used to;
a) Reduce Fever:
Paracetamol is approved for reducing fever in people of all ages. The World Health Organization (WHO) recommends that paracetamol only be used to treat fever in children if their temperature is greater than 38.5 °C (101.3 °F). The efficacy of paracetamol by itself in children with fevers has been questioned and a meta-analysis showed that it is less effective than ibuprofen. Paracetamol has a well-established role in pediatric medicine as an effective analgesic and antipyretic.
b) Reduce Pain:
Paracetamol is used for the relief of pains associated with many parts of the body. It has analgesic properties comparable to those of aspirin, while its anti-inflammatory effects are weaker. It is better tolerated than aspirin in patients in whom excessive gastric acid secretion or prolongation of bleeding time may be a concern. Available without a prescription, it has in recent years increasingly become a common household drug.
1.2.2 PARACETAMOL INDUCED HEPATOXICITY
The toxic effect of paracetamol on the liver occur when the liver enzymes catalyzing the normal conjugation reactions are saturated, causing the drug to be metabolized by the mixed functio
You either get what you want or your money back. T&C Apply
You can find more project topics easily, just search
SIMILAR BIOCHEMISTRY FINAL YEAR PROJECT RESEARCH TOPICS
1. AMELIORATING ROLE OF N.P.K. FERTILIZER ON THE TOXIC EFFECTS OF Ni ON (SORGHUM) ROOT ANTIOXIDANT ENZYMES» ABSTRACT This study investigated the activities of superoxide dismutase (SOD), catalase (CAT), glutothione peroxidase (GP) and the level of malondiald...Continue Reading »
2. ANTISICKLING EFFECT AND ACTIVITY GUIDED FRACTIONATION OF DICHROSTACHYS CINEREA (Wight & Arn) ROOT AND STERCULIA SETIGERA (Del) LEAF METHANOLIC EXTRA...» ABSTRACT The in vitro antisickling effects of the crude methanolic extract, ethyl acetate, n hexane, butanol, and aqueous fractions of methanolic extr...Continue Reading »
3. ASSESSEMENT OF INFANT AND YOUNG CHILD FEEDING PRACTICES AND NUTRITIONAL STATUS OF CHILDREN (0-23 MONTHS) IN FUNTUA LOCAL GOVERNMENT AREA, KATSINA STAT...» ABSTRACT Appropriate feeding practices are essential for the nutrition, growth, development and survival of infants and young children.Assessment of i...Continue Reading »
» CHAPTER ONE 1.0 INTRODUCTION Seed and coat of Mucuna pruriens, known as velvet beans or cowitch has scientific classification as shown below; Kingdom ...Continue Reading »
5. ANTIMICROBIAL AND PHYTOCHEMICAL PROPERTIES OF YOUNGCOCOS NUCIFERA (COCONUT) WATER AND METHALONIC EDXTRACT OF THE YOUNGCOCOS NUCI» CHAPTER ONE 1.0 INTRODUCTION AND LITERATURE REVIEW 1.1 INTRODUCTION Medicinal plants represent a rich source of antimicrobials and many other drugs. T...Continue Reading »
6. EFFECTS OF ETHANOL, METHANOL AND N-HEXANE LEAF AND FRUIT EXTRACTS OF Kigelia africana ON SOME OXIDATIVE AND BIOCHEMICAL PARAMETERS IN ALLOXAN-INDUCED ...» ABSTRACT Globally, the estimated incidence of diabetes and projection for the year 2030 as given by the International Diabetes Federation (IDF) is 350...Continue Reading »
7. ASSESSMENT OF THE RELATIONSHIP BETWEEN FOETAL HAEMOGLOBIN LEVELS, MARKERS OF TISSUE DESTRUCTION AND DISEASE SEVERITY IN INDIVIDUALS WITH SICKLE CELL ...» ABSTRACT Sickle cell Anaemia is a genetic disorder that leads to the production of Sickle haemoglobin, whichresults in abnormal functions of the red b...Continue Reading »
8. EFFECT OF ETHANOLIC ROOT EXTRACT OF JATHROPHA TANJORENSIS ON KIDNEY FUNCTION OF FEMALE ALBINO WISTAR RATS» ABSTRACT Jatropha tanjorensis has been consumed as a leafy vegetable and as a medicinal plant in Nigeria has shown hematological, antimalarial, antimi...Continue Reading »
9. ANTI-HYPERLIPIDEMIC POTENTIAL OF VITEX DONIANAETHANOLEXTRACTS ON POLOXAMER 407 INDUCED HYPERLIPIDEMIC AND NORMAL RATS.» ABSTRACT Anti hyperlipidemic potential of extracts (aqueous, 70% methanol, 70% ethanol and 70%, acetone) of Vitexdoniana leaves, stem bark and root ba...Continue Reading »
10. DIFFERENTIAL EXPRESSION LEVELS OF GLUTATHIONE-S-TRANSFERASE (OvGST1) GENE IN THE HOST (HUMAN) AND VECTOR Simulium damnosum» Abstract Onchocerciasis, a neglected tropical disease (NTD), also known as river blindness, the world‟s second leading cause of preventable blindnes...Continue Reading »