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ABSTRACT
This study evaluated, the antidiarrhoeal and antibacterial activity of the methanolic extracts of
seed and pulp of Ziziphus mauritania. The crude extracts were prepared by maceration of seed
and pulp powder in methanol. The pulp extract was partitioned into n-hexane, ethylacetate, n-
butanol and aqueous fractions. The ethylacetate extract was further fractionated by column
chromatography into four fractions A-D. The antibacterial activity of the crude extracts, and
fractions were evaluated by Agar well diffusion method .The antidiarrhoeal activity of the crude
seed and pulp extracts were evaluated using castor oil-induced diarrheal model, charcoal meal
test and anti-fluid accumulation test in rats. The result of phytochemical test indicated that
tannins, flavonoids, saponin, cyanogenic glycosides, and terpenoids were present in both seed
and pulp extracts. The antibacterial susceptibility test showed that the seed and pulp extracts
possess inhibitory activity against Staphyloccus aureus, Eschericial coli and Salmonella spp.
The fractions obtained from the pulp extract also showed varied antibacterial activity, the
diameter of the zone of inhibition obtained ranged from 29-11 and 27-10 mm for seed and pulp
respectively. In the castor oil-induced model, fluid accumulation test, the methanolic extracts of
the seed and pulp produced a significant (p<0.05) decline in volume of intestinal contents.
Results from the charcoal meal test revealed that all the extract produced a significant (p<0.05)
anti-motility effect. Based on the findings of this work, the pulp methanolic extract of this plant
possess antibacterial and anti-diarrhoeal properties and validates its use in traditional medicine
for the treatment of diarrhoea and a number of ailments caused by microorganisms.
CHAPTER ONE
1.0 INTRODUCTION
1.1 Background of the Study
Since the beginning of human civilization, medicinal plants have been used for their therapeutic
value. Nature has been a source of medicinal agents for thousands of years and an impressive
number of modern drugs have been isolated from natural sources. Many of these isolations were
based on the uses of the agents in traditional medicine. The plant-based, traditional medicine
system continues to play an essential role in health care, with about 80% of the world‟s
inhabitants relying mainly on traditional medicines for their primary health care (Shaikh and
Hatcher, 2005).
According to the World Health Organization “a medicinal plant” is any plant, which in one or
more of its organs contains substances that can be used for the therapeutic purposes or which, are
precursors for the synthesis of useful drugs(WHO, 2001). This definition distinguishes those
plants whose therapeutic properties and constituents have been established scientifically and
plants that are regarded as medicinal but which have not yet been subjected to thorough
investigation. The term “herbal drug” determines the part/parts of a plant (leaves, flowers, seeds,
roots, barks, stems, etc.) used for preparing medicines (Anonymous, 2007). Furthermore, WHO
defines medicinal plant as herbal preparations produced by subjecting plant materials to
extraction, fractionation, purification, concentration or other physical or biological processes
which may be produced for immediate consumption or as a basis for herbal
products(WHO,2004). Medicinal plants are plants containing inherent active ingredients used to
cure disease or relieve pain (Okigboet al., 2005). 1
The use of traditional medicines and medicinal plants in most developing countries as
therapeutic agents for the maintenance of good health has been widely observed (UNESCO,
1998). Modern pharmacopoeia still contains at least 25% of drugs derived from plants and many
others, which are synthetic analogues, built on prototype compounds isolated from plants.
Interest in medicinal plants as a re-emerging health aid has been fuelled by the rising costs of
prescription drugs in the maintenance of personal health and well-being and the bioprospecting
of new plant-derived drugs. The growing recognition for medicinal plants use is due to several
reasons, including escalating faith in herbal medicine (Kala, 2005). Furthermore, an increasing
reliance on the use of medicinal plants in the industrialized societies has been traced to the
extraction and development of drugs and chemotherapeutics from these plants as well as from
traditionally used herbal remedies (UNESCO, 1998).
The medicinal properties of plants could be based on the antioxidant, antimicrobial, antipyretic
effects of the phytochemicals in them (Ayodele, 2005). According to World Health
Organization, medicinal plants would be the best source to obtain a variety of drugs. Therefore,
such plants should be investigated to better understand their properties, safety and efficacy
(Nascimentoet al., 2000).
Medicinal plants produce bioactive compounds used mainly for medicinal purposes. These
compounds either act on different systems of animals including man, and/or act through
interfering with the metabolism of microbes infecting them. The microbes may be pathogenic or
symbiotic. In either way, the bioactive compounds from medicinal plants play a determining role
in regulating host-microbe interaction in favour of the host. So the identification of bioactive
compound in plants, their isolation, purification and characterization of active ingredients in
crude extracts by various analytical methods is important. The instant rising demand of plant-
2
based drugs is unfortunately creating heavy pressure on some selected high-value medicinal
plant populations in the wild due to over-harvesting. Several of these medicinal plant species
have slow growth rates, low population densities, and narrow geographic ranges (Kala et al.,
2006); therefore they are more prone to extinction. Conversely, because information on the use
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